Active Ingredients: Doxycycline
These animals were crossed with mice transgenic for the LacZ gene downstream of a cassette of tet operator TRE binding sites.
A transgenic system for inducible RGC expression has been developed that demonstrates minimal leakiness and significant induction with doxycycline.
This suspension thus obtained was digested with 0.
The pellet obtained after the NaOH digestion step was dried and solubilized with 0. These soluble and insoluble matrix elastin fractions, as well as the tropoelastin fraction in the cell culture medium were then measured using the Fastin assay.
The amounts of elastin measured were also normalized to their corresponding DNA amounts, so as to provide an accurate comparison between the different treatments. After 7 days of culture, as described in Section 2. Maximum volumes of sample protein 15.
Fluoro-luminescent detection of the protein bands was then carried out using a LI-COR Odyssey laser-based scanning system. The normalized band intensities of active MMP-2 for the PLGA NP-treated and other test cultures were further normalized to those of the NP-untreated control cultures to determine the fold-change s in production of active MMP-2, and the statistical significance of the differences between them.
The results presented in this manuscript were averaged from 5 replicate gels run per culture treatment. The gel was run for 2 h at 125 V. The gels were then washed in a buffer containing 2.
The gels were stained with Coomassie Brilliant Blue solution for 45 min, and destained for 90 min, until clear bands appeared visible against the blue background of the gel. Band intensities RDU of the bands obtained for test cultures were measured using ImageJ software, and normalized to those obtained for the NP-untreated control cultures to determine fold changes in MMP-2 activity.
Data was acquired from 3 independent replicate gels. Spatial extent of the cell bodies were defined by labeling filamentous actin using Alex Fluor 488 Phalloidin 1:50 dilution; Invitrogen.
To visualize and confirm the intracellular or extracellular localization of elastin and LOX, z-stack maximum projections overlays were created from images acquired at 0. The dilution of the supernatant due to the addition of solution from the four wash steps was taken into account in the subsequent quantification of NP binding.
The cells were cultured with the NPs for 21 days. The cell layer was harvested at the end of the culture in RIPA buffer, and assayed for LOX activity along with the pooled, spent culture medium.
Le sue opere sono ricche di particolari, quasi invisibili a chi rivolge loro una fugace occhiata, che si rivelano solo allo sguardo attento di coloro che vogliono dedicare parte del loro tempo alla cattura della visione.
In questo non essere simboli indiscutibili, esse chiedono interpretazioni come enigmi. Non evoca, non allude, non rappresenta la parte per il tutto, non contiene il fluire di un tempo e il moto del cambiamento, non esprime trascendenza.
Decontestualizzata, la narrazione ha forma problematica e risponde al dissesto del presente. Giocano seriamente. Adorno, Prisminon. La contemplazione del processo poetico presto ci informa del disincanto e rivela il rischio di una cultura senza senso critico, sterile e autoreferenziale, anestetica.
Testo di Alessandra Santin Endgame, presso la sala esposizioni della biblioteca di Pordenone, gennaio Se inizialmente catturano tutta la nostra attenzione i solitari vasi ricolmi di fiori multicolori, posti in primo piano e in piena luce, dobbiamo poi passare a prendere in considerazione alcuni particolari che rendono queste visioni raffinatamente inquietanti.
Sono immagini simboliche che fanno da contrappunto a queste visioni floreali.